| Annotated protein: | N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (N-acyl phosphatidylethanolamine phospholipase D) (NAPE-PLD) (NAPE-hydrolyzing phospholipase D) (EC 3.1.4.54). Gene symbol: NAPEPLD. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BH82 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NAPEPLD |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic SER membrane |
| Synapse type(s): | Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Nyilas R, et al. "Enzymatic machinery for endocannabinoid biosynthesis associated with calcium stores in glutamatergic axon terminals" J Neurosci. 2008 Jan 30;28(5):1058-63 PMID:18234884 |
| Figure(s): | Figure 3, 4 |
| Annotation description: | "Previous immunogold EM studies have shown that the biosynthetic enzyme of 2-AG is selectively localized to the postsynaptic plasma membrane (Katona et al., 2006; Yoshida et al., 2006). In contrast, the present quantitative analysis of immunogold particle distribution at high resolution found that NAPE-PLD was not associated with the plasma membrane, but rather accumulated on the external surface of intracellular membrane compartments (Fig. 4 A1-B). Furthermore, the majority of gold particles were attached to cisternae of smooth endoplasmic reticulum (SER) (median, 58.08%; range, 52.63-62%; n = 352 gold particles; n = 4 animals) (Fig. 4 C)." Figure 3: "Immunogold particles (arrows) representing the precise subcellular localization of NAPE-PLD are found presynaptically in excitatory axon terminals both in the stratum radiatum (in Schaffer collaterals; b in C) and in the hilus (mossy terminals; b in E), whereas postsynaptic structures [dendritic spines (sp) in C and thorny excrescences (te) in E] are immunonegative." Figure 4: "A, High magnification of serial sections from a mossy fiber terminal demonstrates that NAPE-PLD is present on cisternae of SER, but rarely on synaptic vesicles or on the plasma membrane. B, A similar subcellular localization is found in Schaffer collaterals in CA1. C, Quantitative analysis of subcellular immunogold distribution confirms the preferential localization of NAPE-PLD on cisternae of SER." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5165 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |