| Annotated protein: | Fatty-acid amide hydrolase 1 (EC 3.5.1.99) (Anandamide amidase) (Anandamide amidohydrolase 1) (Fatty acid ester hydrolase) (EC 3.1.1.-) (Oleamide hydrolase 1). Gene symbol: FAAH. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97612 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ FAAH |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | cerebellum |
| Annotated paper: | Gulyas AI, et al. "Segregation of two endocannabinoid-hydrolyzing enzymes into pre- and postsynaptic compartments in the rat hippocampus, cerebellum and amygdala" Eur J Neurosci. 2004 Jul;20(2):441-58 PMID:15233753 |
| Figure(s): | Figure 12 |
| Annotation description: | Figure 4, 10: immunogold-EM of the hippocampus and amygdala showed FAAH (fatty-acid amide hydrolase) localization to dendritic spines but not presynaptic terminals. Figure 12: immunogold-EM of the cerebellum showed (a minor subpopulation of) FAAH labelled axon terminals that formed synapses on FAAH-negative somata and dendrites. Figure 3: FAAH was not detectable in GABAergic interneurons. Figure 1: FAAH antibody specificity was verified by immunostaining hippocampal sections from FAAH-KO mice. Authors discussed the overall localization of FAAH; "As expected from its structure, FAAH may be located on the cytoplasmic surface of membranes. However, quite unexpectedly, the enzyme was primarily associated with intracellular membranes, including smooth endoplasmic reticulum cisternae and the external surface of the mitochondria, but not with the plasma membrane." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5162 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |