| Annotated protein: | E3 ubiquitin-protein ligase Praja-2 (Praja2) (EC 2.3.2.27) (RING-type E3 ubiquitin transferase Praja-2). Gene symbol: PJA2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63364 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PJA2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Nakayama M, et al. "A novel RING-H2 motif protein downregulated by axotomy: its characteristic localization at the postsynaptic density of axosomatic synapse" J Neurosci. 1995 Jul;15(7 Pt 2):5238-48 PMID:7623148 |
| Figure(s): | Figure 7 |
| Annotation description: | Figure 7: "Subcellular localization of Neurodap1 (PJA2) protein by immunoelectron microscopy. Neurodap1-immunoreactivity exists at the postsynaptic density regions of axosomatic synapses (arrows in A and B). Axospinous synapses are negative for Neurodap1 (arrowhead). Punctate Neurodaplimmunoreactivity is present on the cytoplasmic face of ER of neurons (A, C). The immunoreactivity is also seen around the vesicles and sacs of the trans-side of Golgi apparatus (D)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5155 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |