| Annotated protein: | CUB and Sushi multiple domains 2. Gene symbol: CSMD2. Taxonomy: Mus musculus (Mouse). Uniprot ID: V9GX34 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CSMD2 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic density assembly (GO:0097107) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Gutierrez MA, et al. "Csmd2 Is a Synaptic Transmembrane Protein that Interacts with PSD-95 and Is Required for Neuronal Maturation" eNeuro. 2019 May 8;6(2):ENEURO.0434-18.2019 PMID:31068362 |
| Figure(s): | Figure 10, 12 |
| Annotation description: | Figure 10: knockdown of Csmd2 in dissociated hippocampal neurons resulted in reduced dendritic spine density and dendrite complexity. "We next wanted to address whether Csmd2 was important for initial formation of dendritic spines, or their long-term maintenance. To test initial formation, we designed short-term experiments to evaluate the role of Csmd2 in filopodial development 3 d after transfection of dissociated hippocampal neurons at 0 DIV (Fig. 11). We observed a 25% reduction in filopodia density on Csmd2 knock-down at 3 DIV (Fig. 11A,B). Re-introduction of a Csmd2 cDNA that is refractory to the shRNA used brought Csmd2 protein back to control levels and completely rescued filopodia density (Fig. 11B). To examine the role of Csmd2 in dendrite and dendritic spine maintenance, we knocked down Csmd2 expression in neurons at 14 DIV and assessed dendritic spine density and dendrite complexity at 17 DIV (Fig. 12A). Sholl analysis revealed a significant reduction in dendrite complexity in Csmd2 knockdown neurons compared to controls (Figure 12B), which was rescued by restoration of Csmd2 levels. Similarly, shRNA-mediated knockdown of Csmd2 expression resulted in an approximately 60% reduction in dendritic spine density, which was partially rescued by re-introduction of refractory Csmd2 (Figure 12C). We conclude that Csmd2 is required for the initial formation of dendritic filopodia, as well as the maintenance of dendritic spines and the more mature dendritic arbor." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5154 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |