| Annotated protein: | CUB and Sushi multiple domains 2. Gene symbol: CSMD2. Taxonomy: Mus musculus (Mouse). Uniprot ID: V9GX34 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CSMD2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | forebrain |
| Annotated paper: | Gutierrez MA, et al. "Csmd2 Is a Synaptic Transmembrane Protein that Interacts with PSD-95 and Is Required for Neuronal Maturation" eNeuro. 2019 May 8;6(2):ENEURO.0434-18.2019 PMID:31068362 |
| Figure(s): | Figure 5 |
| Annotation description: | "We used in utero electroporation to introduce expression plasmids into progenitors of excitatory neurons in the cortex (Fig. 5A). We electroporated a myr-tdTomato construct together with an expression plasmid for a GFP-tagged intrabody targeting endogenous PSD-95 (iGFP-PSD-95; Gross et al., 2013), allowing us to visualize dendritic spines and PSDs of excitatory neurons in the mature cortex (Fig. 5A). When combined with Csmd2 immunohistochemistry, we readily found Csmd2 puncta co-localized with PSD-95 at the ends of dendritic spines (Fig. 5B, white arrows). Interestingly, not all PSD-95+ spines displayed detectable Csmd2 signal (Fig. 5B, green arrowheads), indicating some heterogeneity in the presence or levels of Csmd2 at spines." Figure 7, 8: Csmd2 is found in synaptosomal and postsynaptic biochemical fractions and interacts with synaptic scaffold proteins. Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5152 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |