Annotated protein:	PDZ and LIM domain protein 5 (Enigma homolog) (Enigma-like PDZ and LIM domains protein). Gene symbol: PDLIM5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62920
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SynGO gene info:	SynGO data @ PDLIM5
Ontology domain:	Biological Process
SynGO term:	regulation of postsynapse assembly (GO:0150052)
Synapse type(s):	hippocampus, glutamatergic
Annotated paper:	Herrick S, et al. "Postsynaptic PDLIM5/Enigma Homolog binds SPAR and causes dendritic spine shrinkage" Mol Cell Neurosci. 2010 Feb;43(2):188-200 PMID:19900557
Figure(s):	Figure 7
Annotation description:	Figure 1, 2: PDLIM5 (aka. ENH, Enigma Homolog) and SPAR (Spine-Associated RapGAP, gene SIPA1L1) co-immunoprecipitated and interacted in yeast two-hybrid. Figure 7: overexpression of PDLIM5a in cultured hippocampal neurons reduced mature mushroom shaped spines and increased thin filopodia-like structures. In contrast, overexpressing PDLIM5b and a PDLIM5a construct lacking LIM domains 2 and 3 caused a significant increase in dendritic spine head size, suggesting that the PDLIM5a overexpression phenotype was conditional on its binding to SPAR. Knockdown of PDLIM5a had the same effect as overexpression of PDLIM5b and PDLIM5a-ΔLIM. Figure 8: postsynaptic localization of PDLIM5 is increased upon PKC activation in cultured hippocampal neurons at 27DIV.
Evidence tracking, Biological System:	Cultured neurons
Evidence tracking, Protein Targeting:	Over-expression
Evidence tracking, Experiment Assay:	Confocal
Annotator(s):	Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:	Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:	5150
Dataset release (version):	20231201
View annotation as GO-CAM model: