| Annotated protein: | PDZ and LIM domain protein 5 (Enigma homolog) (Enigma-like PDZ and LIM domains protein). Gene symbol: PDLIM5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62920 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PDLIM5 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Herrick S, et al. "Postsynaptic PDLIM5/Enigma Homolog binds SPAR and causes dendritic spine shrinkage" Mol Cell Neurosci. 2010 Feb;43(2):188-200 PMID:19900557 |
| Figure(s): | Figure 4, 8 |
| Annotation description: | Figure 1, 2: PDLIM5 (aka. ENH, Enigma Homolog) and SPAR (Spine-Associated RapGAP, gene SIPA1L1) co-immunoprecipitated and interacted in yeast two-hybrid. Figure 4: Cultured hippocampal neurons at 19DIV were immunostained for endogenous PDLIM5, PSD95 (DLG4), synaptophysin (SYP) and GAD65 (GAD2). PDLIM5 partially co-localized with both pre- and post-synaptic marker proteins SYP and DLG4 but not inhibitory synapse marker GAD65. Figure 8: postsynaptic localization of PDLIM5 is increased upon PKC activation in cultured hippocampal neurons at 27DIV. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5149 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |