| Annotated protein: | E3 SUMO-protein ligase PIAS3 (EC 2.3.2.-) (E3 SUMO-protein transferase PIAS3) (Protein inhibitor of activated STAT protein 3). Gene symbol: PIAS3. Taxonomy: Mus musculus (Mouse). Uniprot ID: O54714 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PIAS3 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic cytosol (GO:0099523) |
| Synapse type(s): | hippocampus, glutamatergic cerebral cortex, glutamatergic |
| Annotated paper: | Conz A, et al. "Super-resolution study of PIAS SUMO E3-ligases in hippocampal and cortical neurons" Eur J Histochem. 2021 Aug 11;65(s1):3241 PMID:34459572 |
| Figure(s): | Figure 2, 4 |
| Annotation description: | Figure 2, 4: cultured hippocampal and cortical neurons were stained for PIAS3, synaptophysin (SYP) and PSD95 (DLG4) and imaged using both confocal and SIM super-resolution microscopy. PIAS3 was mostly localized to the nucleus with some cytoplasmic localization, but also showed partial co-localization with pre- and post-synaptic marker proteins. Detection levels of PIAS3 at the synapse were generally lower than PIAS1. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5144 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |