| Annotated protein: | SUMO-conjugating enzyme UBC9 (EC 2.3.2.-) (RING-type E3 SUMO transferase UBC9) (SUMO-protein ligase) (Ubiquitin carrier protein 9) (mUBC9) (Ubiquitin carrier protein I) (Ubiquitin-conjugating enzyme E2 I) (Ubiquitin-protein ligase I). Gene symbol: UBE2I. Taxonomy: Mus musculus (Mouse). Uniprot ID: P63280 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ UBE2I |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic cytosol (GO:0099523) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Colnaghi L, et al. "Super Resolution Microscopy of SUMO Proteins in Neurons" Front Cell Neurosci. 2019 Nov 1;13:486 PMID:31749687 |
| Figure(s): | Figure 2, 3, S6-S9 |
| Annotation description: | "Next, we studied the localization of Ubc9 in primary neurons with synaptic markers. We co-stained hippocampal neurons with Ubc9 and PSD95 or synaptophysin. We found that Ubc9 partially colocalizes with both pre- and postsynaptic markers, indicating that not only SUMO2/3 but also Ubc9 may be present at the synapse (Figure 2). Quantitative analysis indicated a medium-high colocalization rate for Ubc9 and PSD95 and a low colocalization rate for Ubc9 and synaptophysin (Figures 2I,J). To determine whether Ubc9 co-stains with pre- and postsynaptic markers together with SUMO proteins, we first studied, with two different antibodies, whether SUMO1 could also partially localize with PSD95 and synaptophysin. Similarly to SUMO2/3, we found that SUMO1 is predominately a nuclear protein that partially localizes with the two synaptic markers (Supplementary Figures S6-S8). Quantitative analysis of the colocalization of SUMO1 with PSD95 and synaptophysin indicated a lower colocalization rate compared to SUMO2/3 (Supplementary Figures S7I,J and Table 1). Like SUMO2/3, also SUMO1 partially colocalized with PSD95 and synaptophysin independently of mitochondria (Supplementary Figure S9). We next used another antibody against Ubc9, raised in goat, to determine whether SUMO proteins localized with Ubc9 at PSD95 and synaptophysin positive loci. We confirmed that Ubc9 localizes with both PSD95 and synaptophysin, and it partially does so with SUMO2/3 and SUMO1 (Figures 3, 4)." "To determine whether Ubc9 co-stains with pre- and postsynaptic markers together with SUMO proteins, we first studied, with two different antibodies, whether SUMO1 could also partially localize with PSD95 and synaptophysin. Similarly to SUMO2/3, we found that SUMO1 is predominately a nuclear protein that partially localizes with the two synaptic markers (Supplementary Figures S6-S8). Quantitative analysis of the colocalization of SUMO1 with PSD95 and synaptophysin indicated a lower colocalization rate compared to SUMO2/3 (Supplementary Figures S7I,J and Table 1). Like SUMO2/3, also SUMO1 partially colocalized with PSD95 and synaptophysin independently of mitochondria (Supplementary Figure S9). We next used another antibody against Ubc9, raised in goat, to determine whether SUMO proteins localized with Ubc9 at PSD95 and synaptophysin positive loci. We confirmed that Ubc9 localizes with both PSD95 and synaptophysin, and it partially does so with SUMO2/3 and SUMO1 (Figures 3, 4)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5134 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |