| Annotated protein: | Small ubiquitin-related modifier 1 (SUMO-1) (SMT3 homolog 3) (Ubiquitin-homology domain protein PIC1) (Ubiquitin-like protein SMT3C) (Smt3C). Gene symbol: SUMO1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P63166 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SUMO1 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic cytosol (GO:0099523) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Colnaghi L, et al. "Super Resolution Microscopy of SUMO Proteins in Neurons" Front Cell Neurosci. 2019 Nov 1;13:486 PMID:31749687 |
| Figure(s): | Figure 3, S6-S9 |
| Annotation description: | "To determine whether Ubc9 co-stains with pre- and postsynaptic markers together with SUMO proteins, we first studied, with two different antibodies, whether SUMO1 could also partially localize with PSD95 and synaptophysin. Similarly to SUMO2/3, we found that SUMO1 is predominately a nuclear protein that partially localizes with the two synaptic markers (Supplementary Figures S6-S8). Quantitative analysis of the colocalization of SUMO1 with PSD95 and synaptophysin indicated a lower colocalization rate compared to SUMO2/3 (Supplementary Figures S7I,J and Table 1). Like SUMO2/3, also SUMO1 partially colocalized with PSD95 and synaptophysin independently of mitochondria (Supplementary Figure S9). We next used another antibody against Ubc9, raised in goat, to determine whether SUMO proteins localized with Ubc9 at PSD95 and synaptophysin positive loci. We confirmed that Ubc9 localizes with both PSD95 and synaptophysin, and it partially does so with SUMO2/3 and SUMO1 (Figures 3, 4)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | testing the specificity and sensitivity of twenty-four anti-SUMO MAbs towards monomeric and polymeric SUMO1-4 in dot-blots, immunoblots, immunofluorescence and immunoprecipitation @ PMID:36494414 |
| SynGO annotation ID: | 5132 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |