| Annotated protein: | Dihydropyrimidinase-related protein 1 (DRP-1) (Collapsin response mediator protein 1) (CRMP-1) (Inactive dihydropyrimidinase) (Unc-33-like phosphoprotein 3) (ULIP-3). Gene symbol: CRMP1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P97427 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CRMP1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Yamashita N, et al. "Regulation of spine development by semaphorin3A through cyclin-dependent kinase 5 phosphorylation of collapsin response mediator protein 1" J Neurosci. 2007 Nov 14;27(46):12546-54 PMID:18003833 |
| Figure(s): | Figure 3 |
| Annotation description: | CRMP1 is essential for Sema3A-induced changes in dendritic spine morphology in vitro "To investigate whether CRMP1 is involved in mediating Sema3A-induced changes in spine morphology, we used crmp1+/- and crmp1-/- cultured cortical neurons. In the cultured cortical neurons from crmp1+/- mice, Sema3A treatment increased the density of synapsin I and PSD-95 clusters at actin-rich protrusions on dendritic processes (Fig. 3A,B), consistent with our previous finding with wild-type cultured cortical neurons (Morita et al., 2006). In contrast, Sema3A did not alter the density of clusters of synapsin I and PSD-95 in crmp1-/- cortical neurons (Fig. 3A,B). Sema3A also increased the cluster size of synapsin I and PSD-95 in crmp1+/- neurons (Fig. 3A,B). In the absence of Sema3A, the size of synapsin I and PSD-95 clusters in crmp1-/- neurons was significantly larger than that in crmp1+/- neurons (*p < 0.01)." |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Figure 6: Involvement of Tyr504 phosphorylation of CRMP1 in Sema3A-induced cortical dendritic growth. @ PMID:33449368 |
| SynGO annotation ID: | 5127 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |