| Annotated protein: | Dihydropyrimidinase-related protein 1 (DRP-1) (Collapsin response mediator protein 1) (CRMP-1) (Inactive dihydropyrimidinase) (Unc-33-like phosphoprotein 3) (ULIP-3). Gene symbol: CRMP1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P97427 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CRMP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Yamashita N, et al. "Regulation of spine development by semaphorin3A through cyclin-dependent kinase 5 phosphorylation of collapsin response mediator protein 1" J Neurosci. 2007 Nov 14;27(46):12546-54 PMID:18003833 |
| Figure(s): | Figure 2 |
| Annotation description: | "Immunostaining with anti-CRMP1 and anti-MAP2 antibodies revealed that CRMP1 was distributed in the cell body and dendritic processes (Fig. 2A). CRMP1 was detected at both neuronal shafts and actin-rich protrusions on dendritic processes (Fig. 2B). Double staining with anti-CRMP1 and anti-synapsin I or anti-PSD-95 antibodies showed that CRMP1 was colocalized with both synapsin I and PSD-95 at dendritic protrusions (Fig. 2C,D). To further examine the expression of CRMP1 and its synaptic localization, we prepared synaptosomal and PSD fractions from P15 mouse cortex using sucrose gradient fractionation and Triton/sarcosyl extraction. Immunoblot analysis of anti-synaptophysin and anti-PSD-95 antibodies validated the identification of our preparations of synaptosomal and PSD fractions (Fig. 2E). CRMP1 and synapsin I proteins were similarly enriched in the P2 and synaptosomal fractions. In the PSD fractions, a similar enrichment in PSD-IIT fractions was observed with both CRMP1 and PSD-95 proteins (Fig. 2E)." |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5126 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |