| Annotated protein: | SUMO specific peptidase 1. Gene symbol: SENP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1LVT6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SENP1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Zhang J, et al. "Phosphorylation and SUMOylation of CRMP2 regulate the formation and maturation of dendritic spines" Brain Res Bull. 2018 May;139:21-30 PMID:29425794 |
| Figure(s): | Figure 5 |
| Annotation description: | "SENP1 is the active catalytic domain of the SUMO-specific isopeptidase, we expressed mCherry fluorescent protein tagged SUMO specific isopeptidase SENP1 to facilitate deSUMOylation. SENP1-mCherry was cotransfected with GFP or CRMP2-GFP to hippocampal neurons, then the morphology of dendritic spines and mEPSCs of transfected neurons were examined. As shown in Fig. 5A-D, neurons cotransfected with GFP and SENP1 showed an increase not only in the number of total spines but also in the number of mushroom-shape spines when compared with neurons transfected with GFP only. Moreover, neurons co-transfected with CRMP2 and SENP1 also resulted in an increase in the number of total spines and mushroom-shape spines when compared with neurons transfected with CRMP2 only. Furthermore, the number of total spines in neurons co-expressing CRMP2 and SENP1 was more than that in neurons co-expressing GFP and SENP1, especially in the number of mature dendritic spines. Electrophysiological analysis showed that neurons co-transfected with SENP1 increased both the amplitude and frequency of mEPSCs. Moreover, neurons co-transfected with CRMP2 and SENP1 showed higher amplitude and frequency than neurons co-transfected with GFP and SENP1 (Fig. 5E-G)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Electrophysiology (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5124 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |