| Annotated protein: | Sentrin-specific protease 6 (EC 3.4.22.-) (SUMO-1-specific protease 1) (Sentrin/SUMO-specific protease SENP6). Gene symbol: SENP6. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6P7W0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SENP6 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic cytosol (GO:0099523) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Colnaghi L, et al. "Neuronal Localization of SENP Proteins with Super Resolution Microscopy" Brain Sci. 2020 Oct 25;10(11):778 PMID:33113832 |
| Figure(s): | Figure 2 |
| Annotation description: | "Next, we performed a similar analysis for SENP6. For its localization, we co-stained cultured hippocampal neurons with antibodies against SENP6, MAP2, and synaptophysin. ... We next analyzed the co-localization between SENP6 and synaptophysin with profile analysis using two different antibodies (Figure 2D and Supplemental Figure S2A). We quantified the co-localization by Mander's coefficients and Pearson's correlation coefficients, and we obtained, for both, values between 0 and 0.6, to suggest a partial overlap of signals (Figure 2E,F). We next performed a similar analysis to determine the co-localization of SENP6 with drebrin. We started with confocal microscopy (Figure 2G); next, we performed 3D-SIM analysis (Figure 2H,I and Supplemental Figure S2B) to determine the profile analysis of co-localization (Figure 2J) and Mandel's and Pearson's coefficients (Figure 2K,L). We obtained values similar to SENP6 and synaptophysin co-localization. Overall, these data indicate the co-localization of SENP6 with both presynaptic and post-synaptic markers." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5117 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |