| Annotated protein: | Sentrin-specific protease 1 (EC 3.4.22.-) (SUMO-1 protease 2) (SuPr-2) (Sentrin/SUMO-specific protease SENP1). Gene symbol: SENP1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P59110 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SENP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Colnaghi L, et al. "Neuronal Localization of SENP Proteins with Super Resolution Microscopy" Brain Sci. 2020 Oct 25;10(11):778 PMID:33113832 |
| Figure(s): | Figure 1 |
| Annotation description: | "To determine whether SENP1 colocalizes with synaptic markers, we cultured primary hippocampal neurons. ... we identified an area of neuronal processes and performed 3D-SIM (Figure 1B) [45,46]. This technique bypasses the diffraction limits of optical microscopes to reach a resolution of about 100 nm laterally to match the size of synapses [47,48,49,50]. Profile analysis of single loci suggests that SENP1 and synaptophysin colocalize in neuronal processes (Figure 1C,D). To confirm the co-localization results, we decided to perform a similar experiment using a second SENP1 primary antibody. Similarly to our first analysis, we found that SENP1 can localize with synaptophysin (Supplemental Figure S1A). ... To determine whether SENP1 also localizes with post-synaptic markers, we co-stained neurons with antibodies against SENP1, MAP2, and the post-synaptic marker drebrin [52] (Figure 1G-I). Intensity profiles of 3D-SIM images suggest that, in neuronal processes, SENP1 can also localize with drebrin (Figure 1J). We confirmed the results using a second antibody against SENP1 (Supplemental Figure S1B)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | The synaptic level of endogenous SENP1 is regulated upon mGlu5R activation. The post-synaptic regulation of protein sumoylation is maintained by SENP1 levels @ PMID:30904951 |
| SynGO annotation ID: | 5116 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |