| Annotated protein: | NCK interacting protein with SH3 domain. Gene symbol: NCKIPSD. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZWX4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NCKIPSD |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Cho IH, et al. "SPIN90 phosphorylation modulates spine structure and synaptic function" PLoS One. 2013;8(1):e54276 PMID:23342115 |
| Figure(s): | Figure 1 |
| Annotation description: | "To assess the mechanism underlying its synaptic targeting, we performed immunocytochemical assays on GFP-SPIN90-transfected hippocampal neurons. Anti-SPIN90 antibody clearly stained GFP-SPIN90 spots, as revealed by an exact match between GFP signals and signals generated by immunostaining with anti-SPIN90 antibody (Fig. 1A). Moreover GFP-SPIN90 accumulated at spines in association with the postsynaptic marker, PSD95, and adjacent to the presynaptic marker, Vamp2 (Fig. 1B)" Figure 4, 5: SPIN90 (NCKIPSD) phosphorylation increased its postsynaptic localization and binds PSD95 (DLG4) and Shank with increased efficiency. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Identification of SPIN90-Shank binding regions & SPIN90 promotes Shank1b-mediated spine enlargement @ PMID:19302483 SPIN90/WISH localizes to dendritic spines in cultured neurons and interacts with PSD-95. Overexpression of SPIN90/WISH induces dendritic spine formation by an N-WASP-independent mechanism. Knock down of endogenous SPIN90/WISH by small interfering RNA inhibits dendritic spinogenesis @ PMID:16990791 |
| SynGO annotation ID: | 5113 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |