| Annotated protein: | PH and SEC7 domain-containing protein 3 (Exchange factor for ADP-ribosylation factor guanine nucleotide factor 6 D) (Exchange factor for ARF6 D) (Pleckstrin homology and SEC7 domain-containing protein 3). Gene symbol: PSD3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q2PFD7-3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PSD3 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Fukaya M, et al. "Distinct subcellular localization of alternative splicing variants of EFA6D, a guanine nucleotide exchange factor for Arf6, in the mouse brain" J Comp Neurol. 2016 Sep 1;524(13):2531-52 PMID:27241101 |
| Figure(s): | Figure 3, 8 |
| Annotation description: | Authors generated various antibodies against EFA6D (aka. EFA6R, gene PSD3) isoforms. Figure 3: biochemical fractions showed EFA6D1b/c was localized to the LP1 fraction where PSD marker protein PSD95 (DLG4) was enriched (but note that these are not highly-stringent PSD fractions) and not presynaptic marker protein synaptophysin (SYP). Conversely, synaptophysin was enriched in LP2 fractions where EFA6D1b/c was absent. Figure 8: pre-embedding immunoelectron microscopic analysis in the hippocampal CA3 region showed the pan-antibody detected EFA6D in dendritic spines, dendrites and cell body. "Notably, immunolabeling was hardly detected in the postsynaptic density of dendritic spines by either pre-embedding (Fig. 8A,B,E,F,I,J) or postembedding (data not shown) immunoelectron microscopy with anti-EFA6D1/2a-d, anti-EFA6D1/2s, or anti-panEFA6D1 antibodies. ... On the other hand, the labeling density of EFA6D1/2s was similar to that of panEFA6D1, with high density in the dendrite, spine, and cell body of CA3 pyramidal cells (Fig. 8H,L)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Biochemical fractionation (generic) Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | original characterization of PSD3/EFA6R @ PMID:25296758 |
| SynGO annotation ID: | 5112 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |