| Annotated protein: | Rho GTPase-activating protein 33 (Rho-type GTPase-activating protein 33) (Sorting nexin-26) (Tc10/CDC42 GTPase-activating protein). Gene symbol: ARHGAP33. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80YF9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARHGAP33 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kim Y, et al. "SNX26, a GTPase-activating protein for Cdc42, interacts with PSD-95 protein and is involved in activity-dependent dendritic spine formation in mature neurons" J Biol Chem. 2013 Oct 11;288(41):29453-66 PMID:24003235 |
| Figure(s): | Figure 5, 6, 7 |
| Annotation description: | "To investigate the effects of SNX26 on dendritic spine formation, cultured hippocampal neurons at DIV 13 were co-transfected with GFP or GFP-SNX26 and with mCherry empty vector (23) and then fixed at DIV 14. SNX26 overexpression induced an ~38.2% decrease in total dendritic protrusions. ... To identify which domain(s) of SNX26 may be responsible for the observed effects, neurons were co-transfected with mCherry and various deletion mutants of SNX26. Among the mutants, only the RhoGAP domain showed effects similar to those of SNX26 FL (Fig. 6), indicating that the RhoGAP domain is responsible for the effects of SNX26 on dendritic spine formation. This was confirmed by results from a GAP-defective mutant of SNX26 (R350I), which showed a comparable level of dendritic spine development to that in the control group (Fig. 7)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5111 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |