| Annotated protein: | Palmitoyltransferase ZDHHC8 (EC 2.3.1.225) (Zinc finger DHHC domain-containing protein 8) (DHHC-8). Gene symbol: ZDHHC8. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q5Y5T5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ZDHHC8 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Mukai J, et al. "Palmitoylation-dependent neurodevelopmental deficits in a mouse model of 22q11 microdeletion" Nat Neurosci. 2008 Nov;11(11):1302-10 PMID:18836441 |
| Figure(s): | Figure 3, S1 |
| Annotation description: | "We then collected primary neurons from homozygous and heterozygous knockout mice that lacked the gene encoding Zdhhc8 (Zdhhc8-/- and Zdhhc8+/-), as well as their WT (Zdhhc8+/+) littermates11. We found that compared to neurons from WT mice (n = 12), the number of mushroom spines (but not other spine morphotypes or filopodia) in Zdhhc8+/- and Zdhhc8-/- neurons were reduced by 40% (n = 12, P < 0.0001) and 43(n = 12, P < 0.0001), respectively. ... In control experiments, we confirmed that the observed reduction in spine density and number of excitatory synaptic contacts is a direct effect of reduced Zdhhc8 PAT activity (see Suppl. Data, Suppl. Figs. 1b and 3)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | DHHC8 palmitoylates PICK1. Cerebellar LTD in cultured PNs depends on DHHC8 catalytic activity, and palmitoyl-PICK1 is a key DHHC8 substrate that controls LTD @ PMID:24068808 |
| SynGO annotation ID: | 5109 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |