| Annotated protein: | Palmitoyltransferase ZDHHC8 (EC 2.3.1.225) (Zinc finger DHHC domain-containing protein 8) (DHHC-8). Gene symbol: ZDHHC8. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q5Y5T5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ZDHHC8 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Thomas GM, et al. "Palmitoylation by DHHC5/8 targets GRIP1 to dendritic endosomes to regulate AMPA-R trafficking" Neuron. 2012 Feb 9;73(3):482-96 PMID:22325201 |
| Figure(s): | Figure 1, 2 |
| Annotation description: | Figure 1: DHHC8 specifically binds and palmitoylates GRIP1 (pulldowns for various mutants in HEK293T cells). Figure 2a,b: synaptic localization of DHHC8 was shown by co-localization with Bassoon (BSN) in immunofluorescence imaging of primary hippocampal neurons. Figure 2c: in biochemical fractions, DHHC8 was enriched in PSD fractions. Figure 2g,h: DHHC8 Palmitoylated GRIP1 in primary neurons. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "In primary developing hippocampal neurons transfected with ZDHHC8, we localized immunoreactivity to a perinuclear domain (data not shown), as well as to vesicle-like clusters along the dendritic shafts (Fig. 3c)." @ PMID:15184899 DHHC8 palmitoylates PICK1. Cerebellar LTD in cultured PNs depends on DHHC8 catalytic activity, and palmitoyl-PICK1 is a key DHHC8 substrate that controls LTD @ PMID:24068808 |
| SynGO annotation ID: | 5107 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |