| Annotated protein: | Palmitoyltransferase ZDHHC2 (EC 2.3.1.225) (Acyltransferase ZDHHC2) (EC 2.3.1.-) (Zinc finger DHHC domain-containing protein 2) (DHHC-2). Gene symbol: ZDHHC2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9JKR5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ZDHHC2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor diffusion trapping (GO:0150054) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Noritake J, et al. "Mobile DHHC palmitoylating enzyme mediates activity-sensitive synaptic targeting of PSD-95" J Cell Biol. 2009 Jul 13;186(1):147-60 PMID:19596852 |
| Figure(s): | Figure 3, 6, 7 |
| Annotation description: | Figure 3: DHHC2 (ZDHHC2) antibody was validated by siRNA DHHC2-knockdown and westernblots. DHHC2 and PSD95 (DLG4) were enriched in PSD biochemical fractions. Confocal imaging showed DHHC2 localized to dendritic puncta and the cell body. Figure 6: TIRFM imaging revealed that more DHHC2 was recruited near the membrane upon Kyn or TTX treatment (Fig. 6, B and C; and Video 4), where PSD-95 localized (Fig. S4 A). This translocation was activity sensitive as it was reversible upon washing out of Kyn (Fig. 6 D). Furthermore, we found that Kyn or TTX steadily induced colocalization of endogenous DHHC2 with PSD-95 over 48 h (Fig. 6, E and F). Figure 7: Activity-sensitive PSD-95 palmitoylation by DHHC2 is necessary for homeostatic increase of AMPARs. |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5106 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |