| Annotated protein: | Paralemmin-1 (Paralemmin). Gene symbol: PALM. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q920Q0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PALM |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Arstikaitis P, et al. "Paralemmin-1, a modulator of filopodia induction is required for spine maturation" Mol Biol Cell. 2008 May;19(5):2026-38 PMID:18287537 |
| Figure(s): | Figure 1, 2, 3, 8 |
| Annotation description: | Figure 1a: Immunocytochemical staining of cultured hippocampal neurons reveals that paralemmin-1 (PALM) is localized in patches along the plasma membrane. It is also detected in dendritic filopodia at 10 days in vitro (DIV 10) and spines in mature neurons (DIV26). Figure 1b,c: Hippocampal neurons were cotransfected at DIV 5 with RFP and either GFP, GFP-paralemmin-S, the short variant of paralemmin-1 lacking sequences encoded by exon 8 (GFP-PALM-S) or GFP-paralemmin-L, the long variant containing sequences encoded by exon 8 (GFP-PALM-L). Quantification of the number of filopodia/100 μm shows that expression of both paralemmin-1 splice variants increase filopodia number. Figure 1d,e: Quantification of the number of filopodia/100 μm shows paralemmin-1 knockdown diminishes the number of filopodia formed, and these effects can be rescued upon expression PALM Res. Figure 2: Long-term expression of paralemmin-1 induces spine maturation. Figure 3: paralemmin-1 splice variants increase GluR1 accumulation in dendritic spines. Figure 8: Activity-induced changes in dendritic protrusions are modulated by paralemmin-1. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5104 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |