Annotated protein:Paralemmin-1 (Paralemmin). Gene symbol: PALM. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q920Q0
antibody wiki:
SynGO gene info:SynGO data @ PALM
Ontology domain:Cellular Component
SynGO term:postsynapse (GO:0098794)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Arstikaitis P, et al. "Paralemmin-1, a modulator of filopodia induction is required for spine maturation" Mol Biol Cell. 2008 May;19(5):2026-38 PMID:18287537
Figure(s):Figure 1
Annotation description:Figure 1a: Immunocytochemical staining of cultured hippocampal neurons reveals that paralemmin-1 (PALM) is localized in patches along the plasma membrane. It is also detected in dendritic filopodia at 10 days in vitro (DIV 10) and spines in mature neurons (DIV26).

Figure 1b,c: Hippocampal neurons were cotransfected at DIV 5 with RFP and either GFP, GFP-paralemmin-S, the short variant of paralemmin-1 lacking sequences encoded by exon 8 (GFP-PALM-S) or GFP-paralemmin-L, the long variant containing sequences encoded by exon 8 (GFP-PALM-L). Quantification of the number of filopodia/100 μm shows that expression of both paralemmin-1 splice variants increase filopodia number.

Figure 1d,e: Quantification of the number of filopodia/100 μm shows paralemmin-1 knockdown diminishes the number of filopodia formed, and these effects can be rescued upon expression PALM Res.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Over-expression
Antibody (detection)
RNAi / shRNA
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:5103
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology