| Annotated protein: | Tripartite motif-containing 47. Gene symbol: TRIM47. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZA22 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TRIM47 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Sharma G, et al. "Activity-regulated E3 ubiquitin ligase TRIM47 modulates excitatory synapse development" Front Mol Neurosci. 2022 Sep 21;15:943980 PMID:36211980 |
| Figure(s): | Figure 5, 6 |
| Annotation description: | Figure 5: Hippocampal neurons (DIV 3) were transduced with lentivirus expressing shRNAs against TRIM47 along with mCherry. Prior to transduction, CAG-GFP was transfected in hippocampal neurons (DIV 2) to visualize the dendritic spine. Confocal imaging at DIV21 showed that knockdown of TRIM47 led to a significant increase of total dendritic spine density. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5102 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |