| Annotated protein: | E3 ubiquitin-protein ligase NEURL1 (EC 2.3.2.27) (Neuralized-like protein 1A) (m-neu1) (m-neuralized 1) (Neuralized1) (RING-type E3 ubiquitin transferase NEURL1). Gene symbol: NEURL1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q923S6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NEURL1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Pavlopoulos E, et al. "Neuralized1 activates CPEB3: a function for nonproteolytic ubiquitin in synaptic plasticity and memory storage" Cell. 2011 Dec 9;147(6):1369-83 PMID:22153079 |
| Figure(s): | Figures 1-7 |
| Annotation description: | Figure 1: Neurl1 is expressed in the adult mouse forebrain and is localized in dendrites and at post-synaptic sites in hippocampal neurons. Figure 3: Overexpression of Neurl1 in the adult hippocampus results in enhanced learning and memory and increased synaptic plasticity. Figure 4: Neurl1 overexpression in the hippocampus increases the number of spines and functional synapses and the number of AMPAR subunits GluA1 and GluA2. Figure 5: Neurl1 interacts with and ubiquitinates CPEB3. Figure 6: Neurl1-dependent ubiquitination and ubiquitin modulate the activity of CPEB3 and increase CPEB3-dependent polyadenylation and translation of GluA1 and GluA2 leading to an increase of their protein levels. Figure 7: Neurl1 and ubiquitin increase spine number by modulating the translational activity of CPEB3. Authors summarize: "Taken together, our data suggest that the ability of Neurl1 to ubiquitinate CPEB3 is a limiting factor and facilitates hippocampal synaptic plasticity and hippocampal-dependent memory by modulating CPEB3 translational activity through ubiquitination with consequent induction of an increase of GluA1 and GluA2 protein levels and the formation of new spines." |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5099 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |