| Annotated protein: | Muskelin. Gene symbol: MKLN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: O89050 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MKLN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic endosome membrane (GO:0098895) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Heisler FF, et al. "Muskelin regulates actin filament- and microtubule-based GABA(A) receptor transport in neurons" Neuron. 2011 Apr 14;70(1):66-81 PMID:21482357 |
| Figure(s): | Figure 7 |
| Annotation description: | Figure 7a-b: Sucrose gradient centrifugation of vesicle-enriched brain lysate (VE) fractions. GABAAR α1 and muskelin-containing vesicles cofractionate with transferrin receptor and Rab-7 containing endosomes (n=3) Figure 7c-d: Muskelin (MKLN1) facilitates degradation of GABAARα1 (GABRA1). DAB signals detected by electron microscopy of hippocampal tissue. Muskelin immunoreactivity labels individual (C, arrow), but not all (crossed arrows) small vesicles. In addition, individual (D, left), but not all (right) multivesicular bodies and/or late endosomes stain positive for muskelin. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5097 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |