| Annotated protein: | Muskelin. Gene symbol: MKLN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: O89050 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MKLN1 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor transport postsynaptic membrane to endosome (GO:0098968) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Heisler FF, et al. "Muskelin regulates actin filament- and microtubule-based GABA(A) receptor transport in neurons" Neuron. 2011 Apr 14;70(1):66-81 PMID:21482357 |
| Figure(s): | Figure 1-7 |
| Annotation description: | Figure 1: muskelin (MKLN1) is partially localized to the postsynaptic sites of inhibitory synapses (but also at e.g. nonsynaptic intracellular vesicles) and can interact with GABAARα1 (GABRA1). Figure 3: Muskelin depletion causes increased GABAARα1 surface levels with accumulation at extrasynaptic sites. Figure 4: Muskelin-myosinVI (MYO6) interactions facilitate F-actin dependent GABAARα1 internalization in WT but not in muskelin and myosinVI mutant mice. Figure 6: Dynein-driven, retrograde transport complex containing muskelin and GABAAR α1. Figure 7: Muskelin is co-localized with GABAARα1 at (late) endosomes and facilitates degradation of GABAARα1. Authors summarize: "our data demonstrate that muskelin acts as a dual component with common functions in two subsequent internalization and degradation steps involving different cytoskeletal elements and motor proteins (Figure 8)." |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) IP + WB/MSMS Y2H Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5096 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |