| Annotated protein: | Muskelin. Gene symbol: MKLN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: O89050 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MKLN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic specialization membrane of symmetric synapse (GO:0099164) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Heisler FF, et al. "Muskelin regulates actin filament- and microtubule-based GABA(A) receptor transport in neurons" Neuron. 2011 Apr 14;70(1):66-81 PMID:21482357 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1a-g: yeast two-hybrid show interaction between muskelin (MKLN1) and GABAARα1 (GABRA1), but not any other GABAAR. Muskelin-GABAARα1 interaction was further confirmed by various pulldowns in vitro and vivo. Figure 1h-j: "Coimmunostaining of hippocampal neurons, cultured for 12-14 days in vitro (DIV12-14) indicated colocalization of muskelin and GABAAR α1 puncta in somata and neurites. Merged puncta were either positive or negative for the presynaptic terminal marker SV2, demonstrating both synaptic and non-synaptic colocalization, respectively (Figure 1H-J)." Figure 1k-l: "Electron microscopy (EM) analysis of muskelin-specific immunoperoxidase signals confirmed this view. Muskelin was identified at post-, but not presynaptic sites, of many but not all symmetric (inhibitory) synapses (Figure 1K, L), as well as at individual nonsynaptic intracellular vesicles." |
| Evidence tracking, Biological System: | Cultured neurons Cell-free system Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal IP + WB/MSMS Y2H |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5095 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |