| Annotated protein: | Connector enhancer of kinase suppressor of ras 2 (Connector enhancer of KSR 2) (CNK homolog protein 2) (CNK2) (Membrane-associated guanylate kinase-interacting protein) (Maguin). Gene symbol: CNKSR2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z1T4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CNKSR2 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of postsynaptic density membrane (GO:0099147) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Zieger HL, et al. "Disease-associated synaptic scaffold protein CNK2 modulates PSD size and influences localisation of the regulatory kinase TNIK" Sci Rep. 2020 Mar 31;10(1):5709 PMID:32235845 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1d-f: immunofluorescence imaging of CNK2 (gene CNKSR2) in cultured primary rat hippocampal neurons at DIV21 showed localization to dendritic spines. Double-labeling showed CNK2 co-localized with PSD-95 (DLG4) and staining was adjacent to presynaptic marker protein synapsin-1 (SYN1). Based on the high quality imaging and because loss of CNK2 reduced PSD size (Fig. 2) and expression of a CNK2 variant that does not bind to the membrane affected PSD morphology (Fig. 3), this protein was annotated as PSD localized. This protein was annotated as "extrinsic to the membrane" based on its attachment to the phospholipid membrane via the PH domain and the C-terminal stretch of the PH domain. Authors confirmed this using a EGFP-tagged CNK2 deletion construct that lacks the C-terminal region including the PH domain (Figure S2). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5093 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |