| Annotated protein: | Afadin (Afadin adherens junction formation factor) (Protein Af-6). Gene symbol: AFDN. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9QZQ1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AFDN |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Maruo T, et al. "Involvement of l-afadin, but not s-afadin, in the formation of puncta adherentia junctions of hippocampal synapses" Mol Cell Neurosci. 2018 Oct;92:40-49 PMID:29969655 |
| Figure(s): | Figures 2-5 |
| Annotation description: | Figure 4: reverse-IPs and mutated-domain-controls showed l-afadin can interact with alphaE-catenin and alphaN-catenin. Figure 2, 3, 5: dendritic puncta for N-cadherin and alphaN-catenin in cultured hippocampal neurons were reduced in afadin (AFDN) deficient mice and could be rescued by overexpressing l-afadin (long isoform) but not s-afadin (short isoform). Overexpressing a mutated construct of l-afadin showed its FAB domain was required (Fig. 3). Disrupting the αN-catenin to l-afadin binding incurs the same phenotype (Fig. 5). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5091 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |