Annotated protein:Connector enhancer of kinase suppressor of ras 2 (Connector enhancer of KSR 2) (CNK homolog protein 2) (CNK2). Gene symbol: CNKSR2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80YA9
antibody wiki:
SynGO gene info:SynGO data @ CNKSR2
Ontology domain:Biological Process
SynGO term:postsynaptic specialization organization (GO:0099084)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Maruo T, et al. "s-Afadin binds to MAGUIN/Cnksr2 and regulates the localization of the AMPA receptor and glutamatergic synaptic response in hippocampal neurons" J Biol Chem. 2023 Apr;299(4):103040 PMID:36803960
Figure(s):Figure 2
Annotation description:Figure 1: as demonstrated by reverse-IP and mutated-domain-controls in the crude membrane fraction prepared from the mouse brains; s-afadin (AFDN) interacts with MAGUIN-1 (CNKSR2, long isoform) and MAGUIN-2 (CNKSR2, shorter isoform that lacks the C-terminal 137 amino acids).

Figure 2, 4: s-afadin (specifically its RA domains) and MAGUIN-1 are required for the localization of PSD-95 (DLG4) in the cultured hippocampal neurons. Further, loss of MAGUIN-1 impaired cell surface localization of the AMPA receptors (Fig. 6).
Evidence tracking, Biological System:Cultured neurons
Intact tissue
Evidence tracking, Protein Targeting:Over-expression
Evidence tracking, Experiment Assay:IP + WB/MSMS
Confocal
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:5090
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology