| Annotated protein: | Afadin (Afadin adherens junction formation factor) (Protein Af-6). Gene symbol: AFDN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O35889 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AFDN |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic specialization organization (GO:0099084) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Maruo T, et al. "s-Afadin binds to MAGUIN/Cnksr2 and regulates the localization of the AMPA receptor and glutamatergic synaptic response in hippocampal neurons" J Biol Chem. 2023 Apr;299(4):103040 PMID:36803960 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 1: as demonstrated by reverse-IP and mutated-domain-controls in the crude membrane fraction prepared from the mouse brains; s-afadin (AFDN) interacts with MAGUIN-1 (CNKSR2, long isoform) and MAGUIN-2 (CNKSR2, shorter isoform that lacks the C-terminal 137 amino acids). Figure 2, 4: s-afadin (specifically its RA domains) and MAGUIN-1 are required for the localization of PSD-95 (DLG4) in the cultured hippocampal neurons. Further, loss of MAGUIN-1 impaired cell surface localization of the AMPA receptors (Fig. 6). |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | IP + WB/MSMS Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5089 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |