| Annotated protein: | Afadin (Afadin adherens junction formation factor) (Protein Af-6). Gene symbol: AFDN. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9QZQ1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AFDN |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone membrane (GO:0048787) |
| Synapse type(s): | Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Mizoguchi A, et al. "Nectin: an adhesion molecule involved in formation of synapses" J Cell Biol. 2002 Feb 4;156(3):555-65 PMID:11827984 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 2c: Adult mouse hippocampal sections were singly stained with rabbit polyclonal anti-l-afadin, followed by immunoelectron microscopy with the silver-enhanced immunogold method. Afadin (AFDN) was detected on both the pre- and post-synaptic plasma membrane facing the synaptic cleft. "It is therefore likely that at puncta adherentia junctions of these synapses, nectin-1 and -3 form a hetero-trans-dimer that is associated with l-afadin on both sides of the junctions." Figure 4b: a similar localization was observed in developing mouse hippocampal sections on P4, P7, and P14. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5087 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |