| Annotated protein: | Actin-associated protein FAM107A. Gene symbol: FAM107A. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q78TU8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ FAM107A |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic actin cytoskeleton (GO:0098871) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Schmidt MV, et al. "Tumor suppressor down-regulated in renal cell carcinoma 1 (DRR1) is a stress-induced actin bundling factor that modulates synaptic efficacy and cognition" Proc Natl Acad Sci U S A. 2011 Oct 11;108(41):17213-8 PMID:21969592 |
| Figure(s): | Figure 4 |
| Annotation description: | Figure 2: DRR1 (FAM107A) Binds to, Stabilizes, and Bundles F-Actin. Figure 3: DRR1 (FAM107A) affects F-actin assembly in vitro. Figure 4c: "To visualize DRR1 (FAM107A) localization at higher resolution in the hippocampal CA3 via electron microscopy, we performed double immunogold labeling together with synaptophysin (SYP). DRR1 was shown to be predominantly presynaptic (Fig. 4C). Analysis of 48 images (total area assessed was 610 μm2) revealed that 62% of the examined giant boutons (n = 112) were positive and colabeled with the presynaptic marker synaptophysin, whereas only 23% of the postsynaptic thorns were DRR1 positive." Note that only a minor portion of DRR1 is synaptic, even within stained neurons; "Furthermore, we also observed a partial overlap with synapsin, which suggests that DRR1 localizes to some, but presumably not all, synapses (Fig. 4B). Accordingly, correlation coefficients vary between 0.1 and 0.8, depending on which subregion of the neurites is analyzed (Fig. S4B)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5085 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |