| Annotated protein: | Elongation factor 1-alpha 2 (EF-1-alpha-2) (Eukaryotic elongation factor 1 A-2) (eEF1A-2) (Statin-S1). Gene symbol: EEF1A2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62632 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EEF1A2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic specialization assembly (GO:0099150) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Becker M, et al. "Effects of two elongation factor 1A isoforms on the formation of gephyrin clusters at inhibitory synapses in hippocampal neurons" Histochem Cell Biol. 2013 Dec;140(6):603-9 PMID:23839781 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 2: eEF1A constructs were generated by PCR amplification of the desired cDNA sequences of rat eEF1A1 and eEF1A2. Overexpressing a control construct, eEF1A1 and eEF1A2 in DIV2 hippocampal neurons enabled observation of the effects of both eEF1A isoform on postsynaptic gephyrin (GPHN) clusters. The effect of both isoforms was very similar, resulting in increased number, size and density of postsynaptic gephyrin clusters. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5079 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |