| Annotated protein: | ENAH, actin regulator. Gene symbol: ENAH. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I5ZT50 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ENAH |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic cytoskeleton organization (GO:0099188) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Bausen M, et al. "The state of the actin cytoskeleton determines its association with gephyrin: role of ena/VASP family members" Mol Cell Neurosci. 2006 Feb;31(2):376-86 PMID:16376568 |
| Figure(s): | Figure 6, 7 |
| Annotation description: | "When hippocampal cultures at DIV 6 were stained for both Mena (gene ENAH) and the presynaptic inhibitory synaptic vesicle protein VIAAT, Mena containing 'hot spots' were clearly observed along dendrites and in neuronal somata (Figs. 6A, A1). Furthermore, although there was a considerable amount of diffusely localized protein, approximately 68% (data not shown) of these Mena puncta colocalized with VIAAT (Figs. 6A, A1). ... Costaining with fluorescently labeled phalloidin showed that Mena puncta were aligned with F-actin in dendrites (Fig. 6A2)." Figure 7: hippocampal neurons treated with cytoskeleton disrupting agent cytochalasin-D showed a strong reduction of gephyrin-VIAAT clusters. Authors conclude: "Our data indicate that Mena (ENAH), an actin uncapping protein that has previously been reported to interact with gephyrin (PMID:12967995), acts as an adaptor that allows for stable gephyrin-actin interactions." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | in Drosophila; "We thus show that Ena, under control of the Dlar pathway, is required for presynaptic terminal morphogenesis and bouton addition and that Ena has active zone and neurotransmission phenotypes. Notably, in contrast to Dlar, Ena appears to integrate multiple pathways that regulate synapse form and function." @ PMID:32183907 Figure 9: "Colocalization of Mena with synaptic structures in cultured spinal cord neurons. Double immunofluorescence for Mena (A, B, green) and gephyrin (A', B', red). The merged images (A", B") show that most, but not all, synapses contain both proteins." @ PMID:12967995 iBioID/BirA proximity labeling showed Mena (gene ENAH) as a major protein at inhibitory synapses @ PMID:27609886 |
| SynGO annotation ID: | 5077 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |