| Annotated protein: | Elongation factor 1-alpha 1 (EF-1-alpha-1) (EC 3.6.5.-) (Elongation factor Tu) (EF-Tu) (Eukaryotic elongation factor 1 A-1) (eEF1A-1). Gene symbol: EEF1A1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62630 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EEF1A1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | forebrain cerebral cortex |
| Annotated paper: | Cho SJ, et al. "Presence of translation elongation factor-1A (eEF1A) in the excitatory postsynaptic density of rat cerebral cortex" Neurosci Lett. 2004 Aug 5;366(1):29-33 PMID:15265584 |
| Figure(s): | Figure 1, 2 |
| Annotation description: | Figure 1: biochemical fractions of rat forebrain homogenate showed eEF1A was enriched in PSD fractions. "When the 'One-Triton' PSD fraction was washed at 4 °C in non-ionic detergents, such as Triton (1.0%) and OG (1.0%), the majority of the eEF1A remained in the pellet fraction. ... Treatment with N-lauroyl sarcosinate (Sarc., 3.0%), a very harsh detergent, extracted the eEF1A almost completely. ... Taken together, these results indicate that eEF1A is associated with the PSD core through ionic interactions." Figure 2: confocal imaging of immunofluorescence labeling for eEF1A (monoclonal antibody against EEF1A1), PSD95 (DLG4, a postsynaptic marker) and SynGAPα (Syngap1, a RasGTPase-activating protein localized exclusively to postsynaptic densities) showed extensive co-localization in rat cultured cortical neurons. "By contrast, doublestaining with α-eEF1A and α-synaptophysin, a presynaptic marker, produced no such colocalization (Fig. 2B, panel c)" |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5075 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |