| Annotated protein: | Serine/threonine-protein kinase BRSK1 (EC 2.7.11.1) (EC 2.7.11.26) (Brain-specific serine/threonine-protein kinase 1) (BR serine/threonine-protein kinase 1) (Serine/threonine-protein kinase SAD-B). Gene symbol: BRSK1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B2DD29 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ BRSK1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle priming (GO:0010807) |
| Synapse type(s): | ganglion, acetylcholinergic hippocampus |
| Annotated paper: | Inoue E, et al. "SAD: a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter release" Neuron. 2006 Apr 20;50(2):261-75 PMID:16630837 |
| Figure(s): | Figures 5-8 |
| Annotation description: | Figure 5: overexpression of a kinase-deficient mutant of SAD-B (BRSK1), where lysine 63 was replaced with arginine, in primary cultured rat hippocampal neurons significantly increased the frequency of mEPSC whereas controls had no effect. "No significant change of its amplitude was observed by overexpression of all the constructs. In addition, no significant difference in the average rise time and decay time of mEPSC was detected (data not shown), suggesting that SAD-B may not be involved in the fusion of SVs." Figure 6: neurotransmission was significantly impaired upon expressing a polyclonal antibody against the SCR2 domain of SAD-B or a recombinant protein of SCR2. Figure 7: SAD-B is involved in control of the readily releasable pool (RRP) size. Figure 8, S7: SAD-B specifically phosphorylates RIM1 but not Munc13-1 at least in vitro. "In summary, we have demonstrated that SAD-B, a novel SV- and active zone cytomatrix-associated protein kinase, is involved in the regulation of neurotransmitter release, perhaps via phosphorylation of the active zone protein and vesicle priming factor RIM1" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Electrophysiology (generic) IP + WB/MSMS Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "Therefore, these data suggest that even after the synaptic responses during LFS reach similar steady-state levels, the replenishment rate of synaptic vesicles from reserve pools, the recycling of released vesicles, or both are significantly slower in SAD-B KO mice. " @ PMID:26444684 |
| SynGO annotation ID: | 5071 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |