| Annotated protein: | Serine/threonine-protein kinase BRSK1 (EC 2.7.11.1) (EC 2.7.11.26) (Brain-specific serine/threonine-protein kinase 1) (BR serine/threonine-protein kinase 1) (Serine/threonine-protein kinase SAD-B). Gene symbol: BRSK1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B2DD29 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ BRSK1 |
| Ontology domain: | Cellular Component |
| SynGO term: | synaptic vesicle (GO:0008021) |
| Annotated paper: | Inoue E, et al. "SAD: a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter release" Neuron. 2006 Apr 20;50(2):261-75 PMID:16630837 |
| Figure(s): | Figure 1, 2, 3 |
| Annotation description: | Figure 2a, 3: immunogold EM showed SAD-B (BRSK1) particles at synaptic vesicles, authors remark partial enrichment at the active zone (but only few EM images are shown and no quantification of puncta). "Note that several signals of SAD-B were also detected in the dendrites (Figure 2A) and cell bodies (data not shown)" Figure 2b,c: SAD-B (BRSK1) was enriched in both the synaptic vesicle fraction and PSD fractions of rat brain homogenate. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Biochemical fractionation (generic) Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5069 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |