| Annotated protein: | Protein argonaute-2 (Argonaute2) (hAgo2) (EC 3.1.26.n2) (Argonaute RISC catalytic component 2) (Eukaryotic translation initiation factor 2C 2) (eIF-2C 2) (eIF2C 2) (PAZ Piwi domain protein) (PPD) (Protein slicer). Gene symbol: AGO2. Taxonomy: Homo sapiens (Human). Uniprot ID: Q9UKV8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AGO2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse maturation (GO:0090128) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Paradis-Isler N, et al. "NMDA receptor-dependent dephosphorylation of serine 387 in Argonaute 2 increases its degradation and affects dendritic spine density and maturation" J Biol Chem. 2018 Jun 15;293(24):9311-9325 PMID:29735530 |
| Figure(s): | Figure 5, 6 |
| Annotation description: | Figure 5: cultured hippocampal neurons, transfected with pEGFP-Ago2WT and pCI-tdTomato and treated with or without NMDA (40 μm NMDA, 15 min), showed that NMDA-induced Ago2 decrease occurs to a greater extent in dendritic spines than in dendrites. Figure 6: "To test whether Ago2 Ser-387 phosphorylation affects spine growth and shape, we expressed Ago2WT, Ago2S387A, or Ago2S387D in cultured hippocampal neurons and quantified dendritic spines on tertiary dendrites. In Ago2WT-expressing neurons, NMDA-R activation was associated with an increase in spine density (Fig. 6A). ... Overall, our results that NMDA-R-dependent spine increase is mimicked by phosphodeficient Ago2 and blocked by phosphomimetic Ago2 support the involvement of Ago2 Ser-387 phosphorylation in modulating NMDA-R-dependent spine growth." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5068 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |