| Annotated protein: | Protein argonaute-2 (Argonaute2) (hAgo2) (EC 3.1.26.n2) (Argonaute RISC catalytic component 2) (Eukaryotic translation initiation factor 2C 2) (eIF-2C 2) (eIF2C 2) (PAZ Piwi domain protein) (PPD) (Protein slicer). Gene symbol: AGO2. Taxonomy: Homo sapiens (Human). Uniprot ID: Q9UKV8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AGO2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Paradis-Isler N, et al. "NMDA receptor-dependent dephosphorylation of serine 387 in Argonaute 2 increases its degradation and affects dendritic spine density and maturation" J Biol Chem. 2018 Jun 15;293(24):9311-9325 PMID:29735530 |
| Figure(s): | Figure 5 |
| Annotation description: | "The ectopic expression of eGFP-Ago2WT together with tdTomato allowed us to compare the effects of NMDA application on Ago2 levels in spines versus dendrites. Similar to dendrites (Fig. 4A), we found that eGFP-Ago2 levels are also decreased in spines upon NMDA-R activation (Fig. 5, A and B). Interestingly, when comparing the turnover of eGFP-Ago2 in spines versus dendrites, we observed a greater decrease of Ago2 in spines compared with dendrites after NMDA-R stimulation (Fig. 5, C and D)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5067 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |