| Annotated protein: | Rho GTPase activating protein 32. Gene symbol: ARHGAP32. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1MAK3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARHGAP32 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Okabe T, et al. "RICS, a novel GTPase-activating protein for Cdc42 and Rac1, is involved in the beta-catenin-N-cadherin and N-methyl-D-aspartate receptor signaling" J Biol Chem. 2003 Mar 14;278(11):9920-7 PMID:12531901 |
| Figure(s): | Figure 2, 3, 4 |
| Annotation description: | Figure 2: biochemical fractions of mouse brain homogenate showed RICS (ARHGAP32) was enriched in the PSD. Figure 3: immunoprecipitation of RICS revealed association with β-catenin, N-cadherin, NMDA receptors, and PSD-95. Figure 4: immunofluorescence labeling and confocal imaging showed RICS co-localized with β-catenin, PSD-95, and NMDA-R in cultured rat hippocampal neurons. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) IP + WB/MSMS Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5066 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |