| Annotated protein: | CXADR-like membrane protein (Adipocyte adhesion molecule) (Adipocyte-specific protein 5) (Coxsackie- and adenovirus receptor-like membrane protein) (CAR-like membrane protein). Gene symbol: CLMP. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R373 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CLMP |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic modulation of chemical synaptic transmission (GO:0099170) |
| Annotated paper: | Jang S, et al. "Clmp Regulates AMPA and Kainate Receptor Responses in the Neonatal Hippocampal CA3 and Kainate Seizure Susceptibility in Mice" Front Synaptic Neurosci. 2020 Dec 21;12:567075 PMID:33408624 |
| Figure(s): | Figure 2, 3, 5, 6, 7 |
| Annotation description: | Figure 2: Subcellular fractionation patterns of Clmp protein in the rat brain showed Clmp is enriched in synaptic, but not PSD, fractions. In HEK293T cells, co-immunoprecipitation of Clmp and PSD-95 (DLG4) showed these proteins do not form a complex (Clmp relatives like IgSF11 are known to interact with PSD-95). Clmp does not have a functional PDZ-binding motif. Figure 3: "Clmp deletion leads to changes in AMPAR, KAR, and NMDAR mEPSCs in Clmp-/- CA3 neurons; increased frequency and amplitude of AMPAR mEPSCs, increased frequency, amplitude, and decay time constant of KAR mEPSCs, and decreased frequency of NMDAR mEPSCs." Figure 5: Extracellular and cytoplasmic regions of Clmp interact with AMPARs and KARs. Figure 6: "However, we detected no genotype difference in LTP at MF-CA3 synapses (Figures 6H,I) or post-tetanic potentiation (PTP) (Figure 6J) of excitatory synaptic transmission at MF-CA3 synapses, as validated by the inhibition of synaptic transmission by the group II mGluR agonist DCG-IV (Figure 6K) and high paired-pulse ratios (Figure 6L). Together, these results suggest that Clmp does not affect presynaptic function or synaptic plasticity at MF-CA3 synapses." Figure 7: Increased extrasynaptic KAR currents and decreased extrasynaptic NMDAR currents in Clmp-/- hippocampal CA3 neurons. |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Biochemical fractionation (generic) IP + WB/MSMS Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5064 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |