| Annotated protein: | Immunoglobulin superfamily member 11 (IgSF11) (Brain and testis-specific immunoglobulin superfamily protein) (Bt-IGSF). Gene symbol: IGSF11. Taxonomy: Mus musculus (Mouse). Uniprot ID: P0C673 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ IGSF11 |
| Ontology domain: | Biological Process |
| SynGO term: | trans-synaptic signaling (GO:0099537) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Hayano Y, et al. "IgSF11 homophilic adhesion proteins promote layer-specific synaptic assembly of the cortical interneuron subtype" Sci Adv. 2021 Jul 14;7(29):eabf1600 PMID:34261648 |
| Figure(s): | Figure 3, 5 |
| Annotation description: | Trans-synaptic signaling of postsynaptic cell adhesion molecule IgSF11 is necessary for morphological differentiation of chandelier-cell presynaptic boutons on axon initial segments of upper layer 2/3 pyramidal neurons. Figure 3, S4: CRISPR-Cas9-based LOF experiments and homozygous KO chandelier-cells displayed significant reductions in the number and size of presynaptic boutons compared to controls. EM imaging showed that presynaptic boutons from controls were complete synaptic boutons (n = 9 boutons), while approximately 30% of presynaptic boutons in homozygous KOs displayed incomplete synaptic boutons (four incomplete synapses out of 14 boutons) with lower number of synaptic vesicles near the active zone. Figure 5: "Given that IgSF11 proteins have homophilic adhesion activity (38), it is plausible to assume that IgSF11 in uL2/3 PNs serves as a ligand for IgSF11 in ChCs. If this is the case, then removal of IgSF11 from uL2/3 PNs must cause phenotypes of ChC presynaptic boutons similar to those observed in IgSF11 homo KO ChCs. To test this possibility, we created the condition where IgSF11 is deleted in postsynaptic cells but not ChCs by transplanting WT ChC progenitors into homo KO pups (Fig. 5A). Homo KO cortices appeared to exhibit the normal distribution of layer-specific PN markers such as Cux1 (a supragranular/granular PN marker) and Ctip2 (an infragranular PN marker) (55) at a gross level, validating that basic fate specification of PNs properly occurs in the absence of IgSF11 (fig. S8, A and B). As expected, IgSF11 LOF in postsynaptic cells significantly reduced the total number of AIS boutons, the number of boutons per AIS, and the size of AIS boutons in EP21 ChCs compared to the control condition where het KO pups were used as host animals (Fig. 5, B to F). These results suggest that IgSF11 in target layer PNs is necessary for the formation and morphological differentiation of AIS boutons of ChCs." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | IgSF11 proteins have homophilic adhesion activity @ PMID:15795899 |
| SynGO annotation ID: | 5062 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |