| Annotated protein: | Immunoglobulin superfamily member 11 (IgSF11). Gene symbol: IGSF11. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q5U2P2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ IGSF11 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Jang S, et al. "Synaptic adhesion molecule IgSF11 regulates synaptic transmission and plasticity" Nat Neurosci. 2016 Jan;19(1):84-93 PMID:26595655 |
| Figure(s): | Figure 2, 3, 5 |
| Annotation description: | IgSF11 mediates homophilic adhesion in a calcium-independent manner (PMID:15795899). IgSF11 belongs to the CAR subgroup of the CTX family of Ig-like cell-adhesion molecules (Fig. S2a). IgSF11 interacts with PSD-95 and is targeted to excitatory synapses in a PDZ interaction-dependent manner (Fig. 1). "IgSF11 associates with AMPARs, as supported by the co-immunoprecipitation of IgSF11 with AMPARs both in vitro and in vivo (Fig. 2a,b). The apparently weak co-immunoprecipitation between the two proteins may be attributable to the restricted expression of IgSF11 in the DG region, the weak nature of the cis-interaction involving the transmembrane domain of IgSF11, or the restricted subcellular site of the interaction, occurring mainly at synapses but not in biosynthetic pathways. Notably, this interaction appears to promote the synaptic stabilization of AMPARs and AMPAR-mediated synaptic transmission. In support of this hypothesis, we found that IgSF11 knockdown suppressed mEPSCs in cultured neurons and mEPSCs and EPSC_AMPA (but not EPSC_NMDA) in slice culture (Fig. 4), IgSF11 knockdown increased surface mobility and endocytosis of AMPARs (Fig. 5), dominant-negative inhibition of IgSF11 decreased mEPSC amplitude (Fig. 6), and in vivo knockout of IgSF11 by two different methods (gene trap and conventional knockout) decreased mEPSC amplitude, but not frequency (Fig. 8d,e)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5061 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |