| Annotated protein: | Protein ELFN1 (Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1) (Protein phosphatase 1 regulatory subunit 28). Gene symbol: ELFN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8C8T7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ELFN1 |
| Ontology domain: | Biological Process |
| SynGO term: | synapse adhesion between pre- and post-synapse (GO:0099560) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Tomioka NH, et al. "Elfn1 recruits presynaptic mGluR7 in trans and its loss results in seizures" Nat Commun. 2014 Jul 22;5:4501 PMID:25047565 |
| Figure(s): | Figure 1, 2, 3 |
| Annotation description: | Figure 1b: in a series of biochemical fractions from mouse brain, transmembrane protein Elfn1 was enriched in PSD and synaptic membrane fractions. Figure 1j: Double immunofluorescence staining of the hippocampal CA1 and DG regions for Elfn1 and mGluR1a (GRM1) showed partial overlap and confirmed postsynaptic spine localization for Elfn1. Figure 2a: postsynaptic Elfn1 recruits presynaptic mGluR7 (GRM7). Overexpression of GFP-Elfn1 strongly increased mGluR7 intensity in dissociated hippocampal neurons. Figure 2c: Triple immunolabeling of endogenous Elfn1, mGluR7 (GRM7) and Vglut1 in untransfected hippocampal neurons at DIV51. mGluR7 signals overlapped with Vglut1 and were adjacent to Elfn1 signals. Figure 3: Elfn1 binds mGluR7 (GRM7) in knockout controlled reverse-immunoprecipitation. |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "This study identifies a novel mechanism for generating constitutive GPCR activity through a transsynaptic Elfn1/mGluR7 structural interaction" @ PMID:30940718 "we identify the binding domain of mGlu7 to Elfn1, a transsynaptic adhesion protein. We find that N-glycosylation of mGlu7 promotes its interaction with Elfn1, thereby enabling proper localization and stable surface expression of mGlu7 at the presynaptic active zone." @ PMID:32931036 |
| SynGO annotation ID: | 5059 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |