| Annotated protein: | Prolow-density lipoprotein receptor-related protein 1 (LRP-1) (Alpha-2-macroglobulin receptor) (A2MR) (CD antigen CD91) [Cleaved into: Low-density lipoprotein receptor-related protein 1 85 kDa subunit (LRP-85); Low-density lipoprotein receptor-related protein 1 515 kDa subunit (LRP-515); Low-density lipoprotein receptor-related protein 1 intracellular domain (LRPICD)]. Gene symbol: LRP1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q91ZX7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LRP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Nakajima C, et al. "Low density lipoprotein receptor-related protein 1 (LRP1) modulates N-methyl-D-aspartate (NMDA) receptor-dependent intracellular signaling and NMDA-induced regulation of postsynaptic protein complexes" J Biol Chem. 2013 Jul 26;288(30):21909-23 PMID:23760271 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 2b: confocal imaging of immunofluorescence labeling for LRP1, Synaptophysin (SYP) and PSD-95 (DLG4) in cultured cortical neurons from mice showed partial localization of LRP1 to the postsynapse. Figure 2c: immunogold EM for LRP1 in CA1 region of the hippocampus showed particles in the postsynapse, including some at the PSD membrane, whereas no presynaptic localization was observed. Immunogold particles were also found on intracellular membranes, such as lysosomes and endosomes of dendritic compartments. Note that LRP1 was also detected in astrocytes (Fig. 1). Figure 2d: Biochemical analysis of synaptosomes and PSD fractions prepared from cortices of adult LRP1-expressing mice showed the presence of LRP1 in the postsynaptic density, from where it was partly co-purified with PSD-95 and NMDA receptor subunits (Fig. 2D). Figure 2e: LRP1 could be co-precipitated with PSD-95 and the NMDA receptor subunits GluN1, GluN2A, and GluN2B (Fig. 2E, a-c) in immunoprecipitation experiments from soluble synaptosomal membrane preparations. |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5056 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |