| Annotated protein: | Neuronal vesicle trafficking-associated protein 2 (Neuron-specific protein family member 2) (Protein 8.5) (Protein p19). Gene symbol: NSG2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P47759 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NSG2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Chander P, et al. "Neuron-Specific Gene 2 (NSG2) Encodes an AMPA Receptor Interacting Protein That Modulates Excitatory Neurotransmission" eNeuro. 2019 Jan 24;6(1):ENEURO.0292-18.2018 PMID:30680309 |
| Figure(s): | Figure 3, 4, 5 |
| Annotation description: | Figure 3c: co-immunoprecipitation showed NSG2 can interact with AMPAR subunits GluA1 (GRIA1) and GluA2 (GRIA2) in whole-brain lysate of P0 mouse. Figure 4b,c, 5a: whole-cell patch clamp recordings from primary hippocampal neurons with CRISPR knockout of NSG2 showed a decrease in mEPSC frequency (Fig. 4). In contrast, primary hippocampal neurons overexpressing mCherry (control) and NSG2-mCherry showed an increase in mEPSC amplitude (Fig. 5). Figure 4f, 5c, 5e: immunofluorescence confocal imaging of primary hippocampal neurons with CRISPR control and knockout of NSG2 showed a significant reduction in PSD95 (DLG4) fluorescence intensity in neurons expressing NSG2 KO, no change for GluA1 (GRIA1) and some reduction of GluA2 (GRIA2) intensity (Fig 4.). In contrast, primary hippocampal neurons overexpressing mCherry (control) and NSG2-mCherry showed a significant increase in fluorescence intensity for both GluA1 and GluA2 (Fig. 5). Authors conclude: "These data suggest that NSG2 is sufficient to drive increased surface expression of AMPARs into existing functional synapses, and potentially to a small proportion of previously silent synapses." |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5055 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |