| Annotated protein: | Neuronal vesicle trafficking-associated protein 2 (Neuron-specific protein family member 2) (Protein 8.5) (Protein p19). Gene symbol: NSG2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P47759 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NSG2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Chander P, et al. "Neuron-Specific Gene 2 (NSG2) Encodes an AMPA Receptor Interacting Protein That Modulates Excitatory Neurotransmission" eNeuro. 2019 Jan 24;6(1):ENEURO.0292-18.2018 PMID:30680309 |
| Figure(s): | Figure 1, 2, 3 |
| Annotation description: | Figure 1F: Western blotting of mouse whole-brain lysates showed NSG2 has its greatest expression during the perinatal period and is gradually downregulated thereafter, it continues to be expressed even in adult animals (P60; Fig. 1F). Figure 1g,h: confocal images of DIV14-DIV16 hippocampal neurons a portion of endogenous NSG2 (29%) and overexpressed NSG2 (37%) co-localized with postsynaptic marker protein Homer1. Figure 2: confocal images of DIV15 hippocampal neurons showed partial (~40%) co-localization of NSG2 with GluA1 (GRIA1) and GluA2 (GRIA2). Figure 3c: co-immunoprecipitation showed NSG2 can interact with AMPAR subunits GluA1 and GluA2 in whole-brain lysate of P0 mouse. Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5054 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |