Annotated protein:IQ motif containing GTPase activating protein 1. Gene symbol: IQGAP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: G3V7Q7
antibody wiki:
SynGO gene info:SynGO data @ IQGAP1
Ontology domain:Cellular Component
SynGO term:postsynapse (GO:0098794)
Synapse type(s):hippocampus
Annotated paper:Nuriya M, et al. "Phosphorylation-dependent interactions of alpha-Actinin-1/IQGAP1 with the AMPA receptor subunit GluR4" J Neurochem. 2005 Oct;95(2):544-52 PMID:16190873
Figure(s):Figures 1-3
Annotation description:Figure 1,2: yeast two-hybrid and co-immunoprecipitation in HEK293T cells showed IQGAP1 interacts with GluR4 (GRIA4).

Figure 3:
"Myc-tagged full-length α-Actinin-1 and IQGAP1 clones were transfected into low density hippocampal primary neuronal cultures together with GFP-tagged GluR4, and the cellular localization of these proteins was analyzed by immunocytochemistry 2 days after transfection.

GFP-GluR4, myc-α-Actinin-1 and myc-IQGAP1 all showed punctate signals throughout the dendrite, both in the shafts and the putative spines (Fig. 3). Moreover, clusters of GFP-GluR4 overlapped extensively with those of myc-α-Actinin-1 (Fig. 3a) and myc-IQGAP1 (Fig. 3b). Co-localizations patterns were even clearer with the surface population of GFP-GluR4 that was specifically labeled by live staining."
Evidence tracking, Biological System:Cultured neurons
Non-neuronal tissue
Cell-free system
Evidence tracking, Protein Targeting:Over-expression
Evidence tracking, Experiment Assay:Confocal
Y2H
IP + WB/MSMS
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:5050
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology